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vendredi 12 octobre 2012

HPLC Troubleshooting



1. INTRODUCTION

Locating and Correcting the Problem

Prevention

2. ABNORMAL PRESSURE

A. No Pressure Reading, No Flow

B. No Pressure Reading, Flow Is Normal

C. Steady High Pressure

D. Steady Low Pressure

E. Pressure Climbing

F. Pressure Dropping to Zero

G. Pressure Dropping, But Not to Zero

H. Pressure Cycling

3. LEAKS

A. Leaky Fittings

B. Leaks at Pump
C. Injector Leaks

D. Column Leaks

E. Detector Leaks

4. PROBLEMS WITH THE CHROMATOGRAM

A. Peak Tailing

B. Peak Fronting

C. Split Peaks

D. Distortion of Larger Peaks

E. Distortion of Early Peaks

F. Tailing, Early Peaks More Than Later Ones

G. Increased Tailing as k Increases

H. Acidic or Basic Peaks Tail

I. Extra Peaks

J. Retention Time Drifts

K. Abrupt Retention Time Changes

L. Baseline Drift

M. Baseline Noise (Regular)

N. Baseline Noise (Irregular)

O. Broad peaks

P. Loss of Resolution
Q. All Peaks Too Small

R. All Peaks Too large

5. PROBLEMS WITH THE INJECTOR

A. Manual Injector, Hard to Turn

B. Manual Injector, Hard to Load

C. Autoinjector,Will Not Turn

D. Autoinjector, Other Problems

6. PROBLEMS DETECTED BY SMELL, SIGHT, OR SOUND

A. Solvent Smell

B. “Hot” Smell

C. Abnormal Meter Readings

D. Warning Lamps

E. Warning Buzzers

F. Squeaks and Squeals

7. KEY PROBLEM AREAS AND PREVENTIVE MAINTENANCE

A. Reservoir

B. Pump

C. Injector

D. Column

E. Detector

F. Genera
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