essential oil extracts from Matricaria pubescens

Distillation of essential oilhttp://www.academicjournals.org/jmpr/PDF/pdf2012/30%20Apr/Makhloufi%20et%20al.pdf

http://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/colloques2/010005535.pdf
The dried aerial parts were grounded before the operation, and
then, 100 g of grounded M. pubescens (Desf.) were submitted to
hydrodistillation for 3 h using a Clevenger apparatus (Amarti et al.,
2008). The distilled essential oils were dried over anhydrous
sodium sulfate, filtered and stored at +4°C until it was used
(Ayoughi et al., 2011; Chanthaphon et al., 2008).

Preparation of ethanolic extractFresh plants were dried in the shade at room temperature and
ground in a coffee bean grinder. 15 g of dried plant material was
soaked in 100 ml of ethanol for 24 h with continuous shaking in a
shaker at room temperature. The plant material was filtered and the
filtrate collected (Abbassi et al., 2005). This was repeated and the
filtrates were combined and concentrated in a rotary evaporator at
30°C to obtain the crude extract and stored at 4°C until further
(Kassi et al., 2008; Iqbal and Arina, 2001).