A Practical Handbook of Preparative HPLC

 

This text is intended to be a guide for both the novice to preparative
HPLC, and as an aid to the chemical engineer planning to introduce this ‘black art’ into the industrial environment.
The first question to ask is ‘What is preparative?’To many, the isolation of a few grams of an extremely potent molecule may be considered as largescale. In some instances 50 g of a vaccine
will supply the annual market for a particular disease state. In more traditional drug therapies a few tonne may be more typical.
The second question to be answered is ‘What is HPLC?’ This abbreviation is often derived from the term ‘High Performance Liquid Chromatography’, though the term ‘High Pressure Liquid
Chromatography’ is often preferred since high performance can also be achieved at low pressure. Just to confuse the issue, is this the pressure created by the resistance to liquid flow through the
column or, the pressure at which the column is packed? To help you to decide whether you have picked up the correct book let’s be practical. This book will describe particles packed
into columns. These stationary phases are rigid porous media  typically in the range of 5–30 m in size and the columns you are interested in are predominantly pre-packed at 2000–6000 psi or
you are going to self-pack your own dynamic axial compression columns at 50–100 bar.
Too many chromatographic texts dwell heavily on a theoretical and mathematical complexity that bears little relevance to what you actually need to do in order to practice preparative HPLC.
Hopefully this book will describe how to practically go about a preparative separation. It is designed to guide the reader through the choice of equipment and chromatographic modes with minimal
fuss and with reference to only relevant formulae. Much of the ‘black art’ will be removed by the hints and tips of a practitioner with over 20 year’s experience in many modes of chromatographic
separation.
Finally, if you know what dynamic axial compression (DAC) is then you have the correct book so read on.

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