Ronald E. Majors, Agilent Technologies, Wilmington Delaware, USA.
`Reversed-phase chromatography is by far the most widely used technique in high
performance liquid chromatography (HPLC).1 It is popular because it is applicable
to most non-polar analytes and to many ionizable and ionic compounds. Most of
the stationary phases used in reversedphase chromatography are hydrophobic in
nature. Therefore, analytes are separated by their degree of hydrophobic interaction
with the stationary phase and matrices with hydrophobic components can also be
retained in a similar manner.
http://www.chromatographyonline.com/lcgc/data/articlestandard//lcgceurope/262003/61538/article.pdf
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